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. 2018 Mar 6;9(2):e02379-17. doi: 10.1128/mBio.02379-17

FIG 5 .

FIG 5 

The persistence of Ab responses against LPS and porins following STmGMMA immunization. CD1 WT mice were immunized i.p. with 1 μg of STmGMMA O-Ag+ (open squares) or O-Ag− (open triangles) at time zero and day 14. (A) B-cell ELISPOT assay used to assess IgG+ ASC against LPS and porins in spleen (left) and BM (right) 14 (top) or 203 (bottom) days after the second immunization. Splenocytes from each mouse were used to seed wells in triplicate at 5 × 105/well. Numbers of spot-forming units (SFU) per million splenocytes are depicted in the graphs, and to the right are representative wells after development. Each dot represents one mouse. (B) ELISA used to analyze titers of IgG against O-Ag (left) or porins (right) in serum samples from mice immunized twice with either STmGMMA O-Ag+ (red circles) or O-Ag− (blue circles) for the time periods indicated. Each circle represents one serum sample. The Mann-Whitney U test with Bonferroni correction for multiple comparisons was used to determine significant differences between groups linked by bars. NI, nonimmunized; NS, not significant;*, P ≤ 0.05; **, P ≤ 0.01.