FIG 1 .
Impact of dietary thiamine on the longevity of conventionally reared (CR) and axenic (Ax) flies. Survival curves of CR and Ax flies on four diets with different vitamin B1 concentrations (0.00, 0.04, 0.2, and 1.0 µg/ml) indicate little difference between treatments. The 95% Wald confidence limits (CL) show significant differences when comparing CR and Ax flies to each other on the 0.04-, 0.2-, and 1.0-µg/ml thiamine diets. Thiamine treatments with the same superscript letters in the key are not significantly different; for example, treatments with the AC label are not significantly different from the treatments with the A or C superscript. The inset table shows the median survival time for the CR and Ax flies. Point estimates and CL are provided in Table S1 in the supplemental material.