Production of thiamine and growth of the bacteria on the no-thiamine diet. (A) Thiamine assay results for the no-thiamine diet inoculated with D. melanogaster microbiota. Box plots represent the amount of thiamine produced on the no-thiamine diet by individual microbiota members or all four in concert. Thiamine concentrations are shown in picomoles per gram of food. (B) CFU recovered for each bacterium per gram of food at 3 days postinoculation. Coculture plots represent the CFU recovered of individual microbiota members from a mixed inoculum of all four microbiota members. Abbreviations: A.p., A. pomorum; A.t., A. tropicalis; L.b., L. brevis; L.p., L. plantarum; All 4, all four microbiota members.