Fig. 2.

Loss of CDK6 kinase activity in mice induced white fat browning. a, e Appearance of male posterior-subcutaneous WAT (sWAT) (a), and dorsal view of NCD-fed WT and K43M mice (e), emphasized with blue squares and arrows. b, f Appearance of a close view of the iWAT (b) and eWAT (f) from the mice indicated. c, g Representative light microscopic images of H&E-stained sections of iWAT (n = 6) and eWAT (n–6) from male mice indicated (scale bars: 100 μm). d, h Representative images of UCP-1 immunohistochemical staining (n = 6) of iWAT and eWAT from mice indicated at 18 weeks of age (scale bars: 100 μm). i, j Relative mRNA expression levels of BAT-specific markers (Ucp-1, Pgc-1α, Cidea, and Prdm16) and WAT-specific markers (Ap2, adiponectin-AdipoQ, and Leptin) of iWAT (i) and eWAT (j) tissues from WT and K43M mice. Data shown are mRNA fold change normalized to the control WT, which is arbitrarily set to 1 unit. k Relative expression levels of mtDNA of eWAT and iWAT from WT and K43M mice. Data shown are fold change of mtDNA compared to control. For i and j, *p < 0.05, vs WT (n = 6), t-test. i Immunoblots of the indicated protein levels in iWAT and eWAT from 50 μg of cell lysates of WT and K43M mice at 18 weeks of age. α-Tubulin is used as an internal loading control. See also Supplementary Figs. 2 and 3