Fig. 4.

Re-expression of CDK6 in mature adipocytes of KO mice reverses white fat browning. Loss of kinase activity in mature adipocytes preserves the effect of loss of kinase activity in germline on white fat browning. a Appearance of male (18 weeks of age) posterior-sWAT of NCD-fed WT, WT-A, KO, and K43M-A, emphasized with blue squares and arrows. b Appearance of isolated iWAT from the mice indicated in a. c Representative light microscopic images of H&E-stained sections of iWAT (n = 6) from male (18 weeks of age) mice indicated in a (scale bars: 100 μm). d Representative images of UCP-1 staining (n = 6) of iWAT from mice indicated at 18 weeks of age (scale bars: 100 μm). e Relative mRNA expression levels of BAT-specific markers (Ucp-1, Pgc-1α, Cidea, and Prdm16) of iWAT under NCD. Data shown are mRNA fold change normalized to their respective controls, WT or WT-A. *p < 0.05, n = 6, t-test, comparing experimental group vs its control. f Immunoblots of the indicated protein levels in iWAT and eWAT from 50 μg of cell lysates. Twenty micrograms of cell lysates of BAT was used as a positive control for UCP-1 and PGC-1α, and α-tubulin was utilized as an internal loading control. g Ex vivo oxygen consumption of iWAT homogenates from mice. Data are expressed as mean ± S.E., *p < 0.05, n = 6, vs WT, t-test. ^✢p < 0.05, n = 6 vs KO, t-test. See also Supplementary Figs. 3 and 5