Fig. 7.

CDK6 inhibits white fat browning by suppressing RUNX1. a Body weight of age-matched male mice (18-week-old) on NCD. *p < 0.05, (n = 6), vs WT, t-test. ^✢p < 0.05, t-test, K43M;Runx1^−/− vs K43M. b Mass of various fat pads was normalized to body weight of male mice on NCD at 18 weeks of age. No significant changes were observed in the masses of BAT and livers among mice indicated under NCD. Data shown are mean ± S.E. (n = 10 for each group), *p < 0.05, t-test, vs WT. ^✢p < 0.05, t-test, K43M;Runx1^−/− vs K43M. c GTT after 18 weeks on NCD. d ITT after 18 weeks on NCD. For c and d, n = 10 for each group, *p < 0.05, t-test, K43M vs WT, ^♦p < 0.05, t-test, Runx1vs WT, ^✢p < 0.05, t-test, K43M;Runx1^−/− vs K43M, ^◆p < 0.05, t-test, K43M;Runx1^−/− vs WT. e Relative mRNA expression levels of BAT-specific markers and WAT-specific markers of iWAT tissues. Data shown are fold changes of mRNA normalized to the control WT, which is arbitrarily set to 1 unit. *p < 0.05 (n = 6), vs WT control, t-test. ^✢p < 0.05, t-test, K43M;Runx1^−/− vs K43M. f Immunoblots of the indicated protein levels in iWAT from 50 μg of cell lysates of the mice indicated at 18 weeks of age. α-Tubulin is used as an internal loading control. g Ex vivo oxygen consumption of iWAT homogenates from mice indicated. Data are expressed as mean ± S.E., *p < 0.05, vs K43M, t-test. ^✢p < 0.05, t-test, K43M;Runx1^−/− vs K43M. See also Supplementary Figs. 6 and 7