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. 2018 Mar 9;9(3):387. doi: 10.1038/s41419-018-0399-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Western blot and qPCR evaluated the protein expression and mRNA of ELF3 in MHCC-LM3-siELF3-1, MHCC-LM3-siELF3-2, MHCC-LM3-siELF3-3 cells and MHCC-LM3-sicontrol cells. b The colony formation and c CCK-8 assay revealed that down-regulation of ELF3 inhibited MHCC-LM3-shELF3 cells' growth in vitro. d Transwell migration and invasion assays indicated that ELF3 knockdown inhibited cell migration and invasion (magnification ×100, scale bar: 200 μm). Each error bar represents the mean ± SD of three replicate samples. p < 0.05 was considered statistically significant. *p < 0.05, **p < 0.01, and ***p < 0.001 based on the Student's t test