Figure 5.

Effect of calcium channel inhibitors on low-voltage-activated (LVA) and high-voltage-activated (HVA) calcium currents in adult beta cells. (A–F) Representative recordings of global calcium currents observed in adult beta cells before and after of calcium channel inhibitors: mibefradil (1 and 10 µM), NiCl₂ (50 µM), TTA-A₂ (5 and 50 µM), and nifedipine (5 µM), respectively. Protocol: voltage ramp from −80 to +60 mV, 500 ms duration, and 0.5 mV/ms slope. (G) Normalized calcium current after of calcium channel inhibitors: 1 µM mibefradil (n = 5), 10 µM mibefradil (n = 6), NiCl₂ (n = 6), 5 µM TTA-A₂ (n = 5), 50 µM TTA-A₂ (n = 3), and nifedipine (n = 6). *p ≤ 0.05 denotes significant differences compared with cells without inhibitors, paired t-test.