Table 3.

Primer sequences

Species	Lineage	Primer	Sequence	Accession number
Equine	Reference	GAPDH	Forward: AAGAAGGTGGTGAAGCAGGReverse: CTCAGTGTAGCCCAGGATG	NM_001163856.1
	Osteogenesis	ALP	Forward: GGAGTATGAGATGGACGAGReverse: GTAGTGAGAGTGCTTGTGCC	XM_005607380.2
		OCN	Forward: TGGCCCTGACTGCATTCTGReverse: CCCTCCTGCTTGGACATGAA	XM_005610022.1
		OPG	Forward: CCCCCCTTGTCCTGACCACTReverse: CGCCCTTCCTCACATTCG	XM_001916099.4
		BSP	Forward: GAAGAATCGGACGCTGAGReverse: ATCGTAGACAGGGTGGTG	XM_001496125.4
Murine	Reference	GAPDH	Forward: ACCCAGAAGACTGTGGATGGReverse: ACACATTGGGGGTAGGAACA	XM_017321385.1
	Osteogenesis	ALP	Forward: TGATGTGGAATACGAACTGGReverse: TGGGAATGCTTGTGTCTG	XM_006538500.2
		OCN	Forward: CCATCTTTCTGCTCACTCTGReverse: TTATTGCCCTCCTGCTTG	NM_007541.3
		OPG	Forward: CACTCGAACCTCACCACAGAReverse: GCTCGATTTGCAGGTCTTTC	NM_008764.3
		BSP	Forward: CAGCCATGAGTCAAGTCAGCReverse: CTTGTGGCTCTGATGTTCCA	NM_001204203.1

ALP alkaline phosphatase, BSP bone sialoprotein, GAPDH glyceraldehyde 3-phosphate dehydrogenase, OCN osteocalcin, OPG osteoprotegerin