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. Author manuscript; available in PMC: 2018 Mar 10.
Published in final edited form as: Nature. 2016 Nov 16;539(7630):583–587. doi: 10.1038/nature20562

Extended Data Table 2.

Cellular complementation and mutation rates of MMR proteins

Strain MMR proteins in
expression
plasmids* 		Genotype Mutation rates†
(per 109 cells)		 Range
(per 109 cells)
MG 1655 - wild type 5.2 1.8–9.0
MG 1655 ΔmutS - mutS− 522.0 334.9–860.8
MG 1655 ΔmutS EcMutS (D835R, R840E)-his6-ald6 MutS-(D835R, R840E) 1.2 0.4–12.9
MG 1655 ΔmutL - mutL− 1004.9 572.2–3481.1
MG 1655 ΔmutL EcMutL-hiS6-ald6 MutL+ 6.3 0.4–9.5
MG 1655 ΔmutL EcMutL (R95F)-his6-ald6 MutL (R95F) 702.3 29.5–1981.3
MG 1655 ΔmutL ald6-his6-EcMutL MutL+ 0.8 0.4–1.2
MG 1655 ΔmutH - mutH− 100.2 77.8–371.9
MG 1655 ΔmutH ald6-his6-EcMutH MutH+ 3.3 1.2–5.7
*

Amino acid substitutions are indicated in parenthesis; the arrangement of the hexa-histidine (his6) and formylglycine-generating enzyme (ald6) recognition sequence relative to the N terminus (beginning) or C terminus (end) of the MMR gene.

†

The median mutation rates are reported.