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. 2018 Mar 9;9:61. doi: 10.1186/s13287-018-0797-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Pluripotency for derived colonies confirmed via immunofluorescence. a Undifferentiated human iPSCs cultured in colonies on vitronectin-coated plates until induction of differentiation (scale bar = 50 μm). b Immunofluorescence showing DNA (green) and positive pluripotent marker expression (red) of TRA1–60, SOX2, OCT4, TRA1–81, and SSEA-4. Yellow indicates costaining of green nuclei and red pluripotent markers (scale bar = 50 μm)