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. 2018 Mar 9;9:55. doi: 10.1186/s13287-018-0781-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Human amniotic mesenchymal stem cells (hAMSCs) improve the proliferation rate of human granulosa cells (hGCs) and upregulate the expression of hGC markers. a A schematic overview of the hGC filter procedures. b The number of ki67⁺AMH⁺ hGCs was evaluated after a co-culture with hAMSCs. c The expression level of the ki67⁺FSHR⁺ hGCs was tested after a co-culture with hAMSCs. d The expression level of the ki67⁺FOXL2⁺ hGCs was tested after a co-culture with hAMSCs. e The number of ki67⁺CYP19A1⁺ hGCs was evaluated after a co-culture with hAMSCs. The experiments were carried out after 7 days of co-culture, n = 3; error bars indicate SD. **p < 0.01, ***p < 0.001, versus the NOA group. AMH anti-mullerian hormone, FOXL2 forkhead box L2, FSHR follicle-stimulating hormone receptor, NOA natural ovarian aging, PBS phosphate-buffered saline