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. 2018 Mar 9;9:55. doi: 10.1186/s13287-018-0781-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — A combination of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) improves proliferation and inhibits the apoptosis level to a greater degree than using them alone after a co-culture with human granulosa cells (hGCs). a A schematic diagram of the EGF and HGF co-culture with hGCs. b A combination of EGF and HGF improved proliferation in the hGCs more significantly than using them singly. c A combination of EGF and HGF inhibited apoptosis in the hGCs more effectively than using them singly. All the experiments were repeated three times; error bars indicate SD. **p < 0.01, ***p < 0.001, versus the HGF group; ^##p < 0.01, ^###p < 0.001, versus the EGF group. NOA natural ovarian aging, PBS phosphate-buffered saline