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. 2018 Mar 9;49:29. doi: 10.1186/s13567-018-0525-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunoprecipitation analysis. LMH cells were infected with FAdV-4 or transfected with pcDNA3.1-F2. Then, 3C2 was used as the capture antibody to perform immunoprecipitation, followed by Western blot analysis using chicken sera against FAdV-4. A Lanes 1 and 2: lysates of LMH cells infected with or without FAdV-4, respectively. Lanes 3 and 4: 3C2-immunoprecipitated pellets of LMH cells infected with or without FAdV-4, respectively. B Lanes 1 and 2: lysates of LMH cells transfected without or with pcDNA3.1-F2, respectively. Lanes 3 and 4: 3C2-immunoprecipitated pellets of LMH cells transfected without or with pcDNA3.1-F2, respectively.