Table 1.

Primers for amplifying the truncated fiber2 gene and the linear pcDNA3.1

PCR product	Primer sequence (5′–3′)
F2_1-415aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTACTATAGCATAGAAG
F2_1-382aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTAGGCTGAACACTTGG
F2_1-349aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTACGTGAGGGTGGCGG
F2_1-316aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTATTGGCGGAGTTGAG
F2_1-283aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTAGGTAGTAGGCGCAA
F2_1-250aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTAGGAGACGCTCCCCC
F2_1-217aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTAGGTCCGCTGGGATC
F2_1-182aa	Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA
	Reverse: ATATCTGCAGAATTTACCTGCGCTTTTAGG
Linear pcDNA3.1	Forward: GAATTCTGCAGATATCCAGCACAGTG
	Reverse: GCTCGGTACCAAGCTTAAGTTTAAACG