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Analysis of HcToll4 expressions in the gills and hepatopancreas from the mussels challenged with S. aureus, V. parahaemolyticus, WSSV, or Poly I:C using qRT-PCR methods. β-actin was used as a reference gene for internal standardization. Each bar represents the value from three independent PCR amplifications and quantifications. Statistical significance is indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001) compared with that of the control.
