Skip to main content
. 2018 Mar 5;9:133. doi: 10.3389/fphys.2018.00133

Figure 6.

Figure 6

Knockdown of HcToll4 or HcToll5 and detection of HcThe and HcWAP expressions. qRT-PCR analysis of the RNA interference efficiency of HcToll4 (A) or HcToll5 (D) in gills of H. cumingii. The experiments were divided into four groups (normal group, 36 h Vibrio challenge group, Vibrio 36 h plus Toll-scrambled-siRNA group, and Vibrio 36 h plus Toll-siRNA group). Expression of HcThe mRNA in mussels after injection with HcToll4-siRNA (B) or HcToll5-siRNA (E) at 36 h. Expression of HcWAP in mussels after injection with HcToll4-siRNA (C) or HcToll5-siRNA (F) at 36 h. Five mussels were used in each group in this assay. Different asterisks indicate significant differences between groups, *P < 0.05, **P < 0.01, ***P < 0.001. The experiment was repeated three times.