Table 1.
E. coli strains from the Keio collection (GE Healthcare Dharmacon) used in this study.
| Pathway | Gene Knockout | Plate-Row-Col | Strain ID |
|---|---|---|---|
| Wild-type | none | ||
|
| |||
| PTS glucose transport | ptsG | 55-G-3 | JW1087-2 |
| crr | 57-H-8 | JW2410-1 | |
|
| |||
| Glucose kinase | glk | 3-C-5 | JW2385-1 |
|
| |||
| Glycogen biosynthesis | pgm | 5-E-5 | JW0675-1 |
|
| |||
| Oxidative pentose phosphate pathway | zwf | 3-C-3 | JW1841-1 |
| ybhE | 19-G-6 | JW0750-3 | |
| gnd | 4-E-3 | JW2011-3 | |
|
| |||
| Non-oxidative pentose phosphate pathway | rpe | 3-C-6 | JW3349-2 |
| sgcE | 73-D-6 | JW4263-1 | |
| rpiB | 4-G-7 | JW4051-2 | |
| tktA | 5-A-6 | JW5478-1 | |
| tktB | 3-F-5 | JW2449-3 | |
| talA | 3-E-5 | JW2448-1 | |
| talB | 1-H-10 | JW0007-1 | |
|
| |||
| Entner-Doudoroff pathway | edd | 51-D-3 | JW1840-1 |
| eda | 51-C-3 | JW1839-1 | |
|
| |||
| Upper glycolysis | pgi | 3-F-7 | JW3985-1 |
| pfkA | 3-F-6 | JW3887-1 | |
| pfkB | 77-B-4 | JW5280-1 | |
| fbp | 5-H-9 | JW4191-1 | |
| fbaB | 77-C-8 | JW5344-1 | |
| tpiA | 4-G-6 | JW3890-2 | |