Table 3.
Goodness-of-fit analysis for individual 13C-MFA fits and for combined analysis of all 14 data sets using COMPLETE-MFA.
| Data set | No. of fitted measurements* |
No. of estimated net free fluxes, exchange fluxes, G-values |
No. of redundant measurements** |
Maximum acceptable SSR*** |
SSR (contribution to SSR in COMPLETE- MFA) |
|---|---|---|---|---|---|
| [1,2]Glc | 66 | 8, 5, 10 | 43 | 63 | 11 (45) |
| [2,3]Glc | 66 | 10, 3, 10 | 43 | 63 | 8 (47) |
| [4,5,6]Glc | 67 | 7, 4, 10 | 46 | 67 | 32 (85) |
| [2,3,4,5,6]Glc | 67 | 9, 5, 10 | 43 | 63 | 31 (71) |
| [1]+[4,5,6]Glc (1:1) | 66 | 7, 4, 9 | 46 | 67 | 11 (35) |
| [1]+[U]Glc (1:1) | 67 | 7, 5, 9 | 46 | 67 | 10 (30) |
| [1]+[U]Glc (4:1) | 67 | 7, 6, 9 | 45 | 65 | 33 (61) |
| [U]Glc (20%) | 67 | 5, 5, 9 | 48 | 69 | 54 (77) |
| [1]Glc | 56 | 7, 6, 8 | 35 | 53 | 6 (54) |
| [2]Glc | 53 | 9, 4, 10 | 30 | 47 | 8 (30) |
| [3]Glc | 51 | 4, 5, 5 | 37 | 56 | 5 (23) |
| [4]Glc | 42 | 2, 2, 0 | 38 | 57 | 15 (20) |
| [5]Glc | 51 | 10, 5, 10 | 26 | 42 | 14 (53) |
| [6]Glc | 53 | 7, 2, 8 | 36 | 54 | 13 (58) |
| COMPLETE-MFA | 813 | 10, 9, 129 | 665 | 738 | 690 |
*
Number of fitted measurements includes mass isotopomers that were non-zero after correction for natural isotope abundances and external flux constraints (i.e. glucose and acetate).
**
The number of redundant measurements equals the number of fitted measurements minus the number of estimated parameters, i.e. net free fluxes, exchange fluxes, and G-values.
***
Maximum acceptable SSR value at 95% confidence level