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. 2017 Nov 1;13(11):1458–1469. doi: 10.7150/ijbs.21300

Figure 5.

Figure 5

Expression of SelH reduced caspase-9 levels in glutamate exposed HT22 cells. (A) Representative immunostaining images from control V-HT22 and SelH-HT22. (B) Representative immunostaining images from glutamate treated V-HT22 and SelH-HT22. (C) Western blot analysis of cleaved and total capsase-9. HT22 neurons were exposed to glutamate for 24 h. The cleaved caspase-9 immunoreactivity (green color) reduced in SelH overexpressed cells under control and glutamate exposure conditions compared with V-HT22. Glutamate significantly increased the protein levels of total capase-9 and cleaved caspase-9 in the cytosolic fraction of the V-HT22 cell. Overexpression of SelH completely blocked the glutamate-induced elevations of total and cleaved caspase-9. **p<0.01 vs. non-glutamate control and ##p<0.01 vs. V-HT22 cell with the same treatment. DAPI (blue color) was used to stain cell the nuclei. Scale bar=125 µm.