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. 2018 Feb 25;2018:7202548. doi: 10.1155/2018/7202548

Figure 3.

Figure 3

Effects of propolin C on PI3K/Akt and ERK-mediated EMT marker expressions in HCC827 lung cancer cells. HCC827 cells were treated with propolin C (2.5, 5, 7.5, and 10 μM) for 24 h. After treatment, the expressions of (a) E-cadherin, vimentin, slug, snail, and β-actin and (b) phospho-ERK, ERK, phospho-Akt, and Akt were analyzed by Western blot as described in Materials and Methods. Significant difference was observed from the control group (∗p < 0.05). (c) HCC827 cells were pretreated with LY294002 (LY, 10 μM) or PD98059 (PD, 10 μM) for 30 min and then incubated with or without propolin C (PPC, 10 μM) for 24 h. After incubation, cells were harvested and Western blot analyses were used to detect E-cadherin, vimentin, snail, and β-actin expressions. Data were shown as mean ± SD (n = 3). Different uppercase letters (A−D) indicate statistical differences among group (p < 0.05), and the same letter showed no difference (p > 0.05).