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. 2018 Feb 25;2018:2353280. doi: 10.1155/2018/2353280

Figure 3.

Figure 3

Separation of sequential compounds according to solvent polarity (apamin, PLA2, and melittin). In total, 13 fractions were isolated from the crude bee venom. Pure melittin was obtained in the 70%–80% ethanol layer.