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. 2018 Mar 6;9:172. doi: 10.3389/fphys.2018.00172

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Copyright © 2018 Liu, Albrecht, Schering, Kuehn, Yang, Zhao and Maak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot detection of bovine ASIP. White boxes indicate specific, blockable bands of recombinant ASIP and the respective size in tissues. The right panel was incubated with antibody previously blocked with the antigen peptide to determine unspecific bindings. Lanes 1 and 12: molecular weight markers, lanes 2–4: 50, 25, 12.5 ng recombinant bovine ASIP after tag removal in PBS, respectively, lanes 5–8: 50 μg of total protein from bovine albumin depleted plasma, subcutaneous fat (SCF), liver, M. longissimus dorsi, respectively, lane 9–10: 50 μg of total protein from bovine SCF and albumin depleted plasma, respectively, after deglycosylation, lane 11: 50 ng recombinant bovine ASIP with SUMO-tag.