Table 1.

Primer sequences for standard PCR.

Primer name	Sequence (5′ 3′)	Genbank acc. no. position	Amplicon length (bp)	Ta (°C)
ASIP Exon2Ca		GK000013.2b	419	62
for	AAATCAACATCTCGGCTTGG	3–22
rev	CTTTTCTGGGTGCCTGATGT	421–402
ASIP genomica		GK000013.2b	430	62
for	AAATCAACATCTCGGCTTGG	3–22
rev	AAAAGGAAAGTGCGGAGGAG	7,089–7,070
MC4R_SNP1		AC_000181.1c	755	57.5
for	AAAATCCTGAATGTTGCCTGG	3,056–3,036
rev	TCCCAATTCAGAGGCAGAAG	2,282–2,301
MC4R_SNP2		AC_000181.1c	601	59
for	TTCTGCCTCTGAATTGGGATTAC	2,300–2,278
rev	GGCTGGGTAGAGTTCATTTTGG	1,700–1,721
MC4R_SNP3		AC_000181.1c	541	58
for	TCGTTTGGGGCAAGTCAAG	1,804–1,786
rev	GATAGTGAAGTACCTGTCCACC	1,264–1,285
MC4R_SNP4		AC_000181.1c	736	59
for	CTCGGTGATCTGTAGCTCCTT	1,339–1,318
rev	CACTCCATGCCCTACACAGA	604–623
MC4R_SNP5		AC_000181.1c	544	57.5
for	TGAAACAGTGCCCAGTCTT	558–540
rev	TAATCCCAAATTGCCTGTGAG	15–35
ATRN_SNP		AC_000170.1d	600	57
for	CAGCGAAACTTGGGGTAGAGT	3,416–3,396
rev	GGCCTTCCAGCTCTAGTCG	2,817–2,835

^aAdopted from Albrecht et al. (2012).

^bNucleotide positions refer to partial sequence of GK000013.2 starting at nt 64,212,950 (i.e., nt 1 in this table = nt 64,212,950).

^cNucleotide positions refer to partial sequence of AC_000181.1 starting at nt 59,670,000 (i.e., nt 1 in this table = nt 59,670,000).

^dNucleotide positions refer to partial sequence of AC_000170.1 starting at nt 52,190,000 (i.e., nt 1 in this table = nt 52,190,000).