Table 2.

Primer sequences used for RT-qPCR.

Primer name	Sequence (5′ 3′)	Genbank acc. no. position	Amplicon length (bp)	Ta (°C)
ASIPa		NM_206843.2	181	60
for	TACCTTGCTGGTCTGCCTGT	200–219
rev	CTTTTCCGCTTCATTTCTGC	380–361
MC1Ra		NM_174108
for	CTGCACTCCCCCATGTACTA	488–507	233	60
rev	ATGGAGATGTAGCGGTCCAC	720–701
MC4Ra		NM_174110.1	163	60
for	CTGATCGGGGTCTTTGTTGT	961–980
rev	GGGCATAAATCAGAGGGTCA	1,123–1,104
ATRN		NM_173995.3	245	60
for	CAGTAATTTCACCTGGCCCATC	3,780–3,801
rev	CATCTGTTTCCAAGGCGACAT	4,024–4,004
B2M		NM_173893	184	60
for	CAGCTGCTGCAAGGATGG	181–204
rev	ATTTCAATCTGGGGTGGATG	417–394
TOP2B		XM_010820383.1	173	60
for	AAGAAAACAGCACCGAAAGG	4,519–4,538
rev	AGGTCTGAGGGGAAGAGGT	4,691–4,673
UXT		NM_001037471	70	60
for	TCATGGCGACGCCCCCTAAAC	30–50
rev	AAAGCCTCGTAGCGCAGCACT	99–79
RPS9		NM_001101152.2	152	60
for	GCTGATCGGCGAGTATGGGCT	146–166
rev	GCCGCCGCAACAGGGCATTA	297–278
FABP3		NM_174313.2	154	60
for	GCGTTCTCTGTCGTCTTTCC	7–26
rev	CTTGGTCATATTGCCCACCT	160–141
FABP4		NM_174314.2	174	60
for	GGATGGAAAATCAACCACCA	354–373
rev	TGGACAACGTATCCAGCAGA	527–508

^aAdopted from Albrecht et al. (2012).