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. 2018 Mar 6;9:162. doi: 10.3389/fpls.2018.00162

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Copyright © 2018 Huang, Guo, Liu, Zhang, Wang, Wang, Wang, Li, Mao, Luan, Liang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis and subcellular localization of OsNCED3. (A) Histochemical staining of Pro_NCED3-GUS transgenic plants, including mature seed (a), bud sheath (b), root (c), leaf (d), culm and node (e), flower (f), stigma (g), pollens(h). Bars = 20 μM. (B) Transcription level of OsNCED3 in different organs. E(embryo), R(root), L(leaf), C(culm), N(node), F(flower). (C) OsNCED3 expression in abiotic stress by qRT-PCR. The three-leaf stage seedlings were performed to 150 mM NaCl, 250 mM Mannitol, 25% PEG and 100 mM H₂O₂ treatment, respectively. The seedling samples were collected for OsNCED3 expression analysis. Data are the mean ± SD for three replicates.