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. 2018 Mar 6;9:162. doi: 10.3389/fpls.2018.00162

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Copyright © 2018 Huang, Guo, Liu, Zhang, Wang, Wang, Wang, Li, Mao, Luan, Liang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPR/Cas9 induced mutation of OsNCED3 and the phenotype of seed germination and early seedling growth in nced3 mutants. (A) Targeted mutagenesis of the OsNCED3 gene. The OsNCED3 mutation site is shown on the gene structure. The sequence in the red box represents the OsNCED3 spacer, and PAM is labeled by underline. The indels are shown in red dashes or letters. (B) Germination rate of wild type and nced3 mutants. Data are the mean ± SD for three replicates (each replicate containing 100 seeds). (C) Growth state at post-germination 2 d, 3 d, and 4 d for wild type and nced3. (D) Root and (E) shoot length of plants post-germination 4 d. (F) Seedling characteristics in the three-leaf stage. (G) Length measurement of (E) seedlings. Data shown are ± SD (n = 14). Similar results in three individual experiments. Asterisks indicate statistically significant differences (Student's t-test; ^*P < 0.05, ^**P < 0.01).