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. 2018 Mar 6;9:285. doi: 10.3389/fpls.2018.00285

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Copyright © 2018 Zhang, Xu and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of SlCNGC1 and SlCNGC14 promoted Xoc-induced and flg22-elicited ROS accumulation. (A) Xoc-induced ROS assay. Hydrogen peroxide (H₂O₂) accumulation at 4 hpi was detected through Diaminobenzidine (DAB) staining analysis. The Xoc-infriltrated leaves before (Up panel) and after (Down panel) staining analyses were presented. (B) flg22-elicited ROS assay. Production of H₂O₂ induced by 100 nM flg22 was detected using a luminol-based assay in leaf disks of SlCNGC1-, SlCNGC14-silenced plants and the eGFP non-silenced control plants. Data are shown as relative luminol units (RLUs) and represent the mean ± SE of three independent experiments.