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. 2018 Mar 6;9:192. doi: 10.3389/fphar.2018.00192

Figure 6.

Figure 6

Calcium signaling mediated by P2X7R and P2Y1R in primary cultures of SCN astrocytes. (A) Increases in intracellular calcium in cultured SCN astrocytes in response to ATP and BzATP (both 50 μM) and partial inhibition of the ATP-induced [Ca2+]i increase by the P2X7R blocker AZ 10606120. The ratio of light intensity (F340/F380) reflects changes in intracellular calcium concentration ([Ca2+]i). (B,C) BzATP-induced [Ca2+]i increases completely (B) or partially (C) inhibited by the P2X7R blocker A438079. (D) Inhibition of ATP-induced responses in the presence of the P2Y1R-selective blocker MRS2179. (E) Concentration-dependent elevation of astrocytic calcium levels by the P2Y1R agonist MRS2365. (F) Concentration-dependent effect of the P2Y2R agonist MRS2768. (G–I) Summary graphs showing the effects of P2 receptor agonists and antagonists on astrocytic [Ca2+]i. Data are presented as the means ± SEM of three to four experiments. **P < 0.01 compared with the control.