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. 2018 Mar 6;12:132. doi: 10.3389/fnins.2018.00132

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Copyright © 2018 Liu, Lu, Iseri, Shi and Kuzum.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Testing setup of the closed-loop system. (A) The diagram of the testing setup. A signal generator was used to apply pulses with different amplitudes and duty cycles into the PBS solution. The analog data was sampled by the closed-loop electrophysiology system and transmitted to the computer for visualization using serial communication. All the recordings were performed in a properly grounded Faraday cage. (B) A picture of the working system. (C) Typical real-time recording data for one channel on the customized software built by MATLAB GUI App designer. A train of 10 Hz 10 ms duration pulses modulated by a 2 Hz sine wave was applied to the saline and the threshold was set to 200 μV. (D) The recorded signal on the software for a train of 20 Hz 5 ms duration pulses modulated by 2 Hz sine wave. The threshold remains the same.