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galK suicide vectors. Maps of the first-generation vector, pMP1267, and the second-generation vector, pMP1269. The unique cloning sites for each are shown, along with the following genes or regions: aph (aphA-1 aminoglycoside phosphotransferase), PL (mycobacteriophage L5 major left promoter), galK (E. coli galactokinase), lacZ (E. coliβ-galactosidase), PmspA (M. smegmatis MpsA porin promoter) and oriE (ColE1 origin of replication).
