Table 1. Bacterial strains used in this study.
| Strain | Description | Source or Reference |
|---|---|---|
| E. coli | ||
| DH10B | F- mcrA Δ(mcrBC-hsdRMS-mrr) [φ80dΔlacZΔM15] ΔlacX74
deoR recA1
endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG |
Invitrogen |
| BW25113 | ∆(araD-araB)567 ∆lacZ4787(:: rrnB-3) lambda- rph-1∆(rhaD-rhaB)568 hsdR514 | CGSC |
| M. smegmatis | ||
| mc2155 | ept-1 | [36] |
| M. abscessus | ||
| ATCC19977 | Clinical isolate, type strain, smooth | ATCC |
| PM3044 | Smooth colony clone of ATCC19977 | This work |
| PM3363 | PM3044 :: pMP1265 (ΔmmpL4b Primary recombinant) | This work |
| PM3365 | PM3363 mmpL4b mutant (Secondary recombinant) | This work |
| PM3366 | PM3363 mmpL4b mutant (Secondary recombinant) | This work |
| PM3386 | PM3044 ΔMAB_2875 | This work |
| PM3389 | PM3044 ΔMAB_2833 :: aacC41 | This work |
| PM3395 | PM3386 ΔMAB_2833 :: aacC41 (ΔMAB_2875 ΔMAB_2833 :: aacC41) | This work |
| PM3404 | PM3386/pMV261 (ΔMAB_2875/VC) | This work |
| PM3405 | PM3386/pMP1270 (ΔMAB_2875/MAB_2875+) | This work |
| PM3492 | PM3044 ΔmbtH | This work |
| PM3498 | PM3492/pMP1294 (ΔmbtH/mbtH+) | This work |