FIGURE 1.

Screens for mutants with defect in bacteriostatic antibiotic induced persister formation. Growth curve of six randomly chosen mutants in 96-well plates, mean and standard error of the mean was shown (A). For the pilot screen, one plate of the Keio library was inoculated to fresh LB broth duplicate, grown for 4 h to log phase and treated for 3 days with 5 μg/ml ofloxacin (B), or pretreated with 100 μg/ml rifampin for 30 min and then added 5 μg/ml ofloxacin for 3 days (C). The plates were then replicated to LB agar and grown overnight at 37°C. The clones with red arrow were considered as positive and selected for further analysis.