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. 2018 Mar 6;9:413. doi: 10.3389/fmicb.2018.00413

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Copyright © 2018 Cui, Niu, Shi, Zhang, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-dependent killing of selected mutants by ofloxacin and static antibiotics in stationary phase culture. Five of the common mutants in the rifampin and tetracycline induced antagonism screens and the parent strain BW25113 were grown overnight and then treated with 5 μg/ml ofloxacin (A), no drug control (B), rifampin (C), and tetracycline (D). At different time points of incubation, bacterial survival was determined by CFU count after removal of the antibiotic by washing. Data represents three independent biological replicates. Error bars represent standard error of the mean (SEM). ^∗Indicates a statistical difference between mutants and parent strain (P < 0.05, t-test).