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. 2018 Mar 6;9:413. doi: 10.3389/fmicb.2018.00413

FIGURE 4.

FIGURE 4

Susceptibility of the mutants to various antibiotics and stress conditions. Stationary phase culture of the mutants and the parent strain BW25113 were treated with ampicillin (A), gentamicin (B), washed and diluted 100-fold to acid pH 4.0 LB broth (C), washed and diluted 100-fold in 0.9% saline for starvation (D), followed by CFU determination on LB agar plates at different time points. Data represents three independent biological replicates. Error bars represent standard error of the mean (SEM). Indicates a statistical difference between mutants and parent strain (P < 0.05, t-test).