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. 2018 Mar 6;9:185. doi: 10.3389/fphys.2018.00185

Figure 4.

Figure 4

Immunolocalization and protein expression of VEGF and HIF in lung tissue. Representative micrographs (40x) showing immunohistochemical distribution of VEGF-A in vascular wall of lung resistance arteries (A), and analysis for VEGF-A vascular immunoreactivity intensity (B). Scale bar: 100 μm. Scanned photograph of immunoblots (C), and HIF-1α (D) and VEGF (E) protein expression. Protein expression was referred to β-actin as control protein. Groups are control (CN, open bars, n = 5) and melatonin treated (MN, closed bars, n = 5) lambs. Values are means ± SEM. Significant differences (P ≤ 0.05): *vs. CN.