Fig. 1.
Amplification analysis of CWD isolates in cervid substrates. (a) Amplification rates of three dilutions of CWD isolate 1 in 95Q/96G/116A/226Q (QGAQ) substrate. The threshold is indicated by the dashed red line. (b) Plotted amplification rates for three dilutions of isolate 1 in QGAQ and canine substrates. The areas under each of these curves were used to summarize the amplification rate data for each substrate/isolate pair. (c) Amyloid extension efficiencies of the 10−1 dilution of isolate 1 in QGAQ and canine substrates. The threshold is indicated by the dashed red line. RFUs, relative fluorescent units; m, slope of the first four hours of the amplification curves after crossing the threshold.