Figure 3.

Expression profiling of the heart-specific miR-34a and mir-208a in peripheral blood from chronic chagasic patients using endogenous miRNAs. Quantitative PCR was performed on total miRNA purified from plasma of normal control individuals (CTR), patients at the indeterminate (IND), and cardiac (CARD) clinical forms of Chagas disease. Individual and median values of relative expression of (A) hsa-miR-208a-3p or (B) hsa-miR-34a-5p normalized by the geometric mean of endogenous non-related hsa-mir-221-3p and hsa-miR-484 expression. Twenty donors, age, and sex matched-individuals were included for each group. Groups were compared by Mann-Whitney non-parametric test and the differences were considered significant at ^*p < 0.05.