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. 2018 Mar 6;11:50. doi: 10.3389/fnmol.2018.00050

FIGURE 6.

FIGURE 6

Topical DEX is not associated with increase in TUNEL-positive cells in injured OE. TUNEL labeling for DNA breaks was used as an index of cell death. Methimazole-lesioned OE did not show increase in TUNEL-positive cells upon the GC treatment (ANOVA F(1,12) < 1 × 10-6, p = 0.9994). We failed to detect changes at 1, 2, or 3 dpl (A–F,I). In order to validate the technic, we used 24 h methimazole single bolus administration as positive control (G,H), which showed several positive nuclei. The cell counting was adjusted to linear extension of OE-selected regions (I). Results represent means ± SEM. Scale bar: 20 μm. White arrows show few TUNEL-positive nuclei.