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Virulence of B. cenocepacia strains determined using the social amoeba Dictyostelium discoïdeum. Strains of Pseudomonas aeruginosa PT5 and Klebsiella pneumoniae KpGe were used as positive and negative control, respectively. The axenic D. discoïdeum strain AX2 was used for virulence assays. Dilutions of amoebae were spotted on bacterial lawn plates and incubated during 5 days (n = 6).
