Fig. 2.

A49 triggers Wnt signalling activity. (a) HEK293T cells were transfected for 24 h with a Wnt-specific luciferase reporter, a control reporter, and TAP-tagged A49 or empty vector (EV). The cells were then stimulated for a further 6 h with Wnt3A-containing medium (black bars) or left unstimulated (white bars). Luciferase activity was calculated and plotted as the mean fold increase ±SD over the unstimulated EV condition. (b) HEK293T cells were transfected as above, as well as with 50 ng per well of a plasmid encoding FLAG-tagged β-catenin (black bars) or EV (white bars). Luciferase activity was calculated 24 h post-transfection as above. (c) U2-OS or HCT116 cells were transfected with TAP-tagged A49 (black bars) or EV (white bars). Luciferase activity was calculated 24 h post-transfection as above. One of at least three independent experiments performed in triplicate is shown in each panel. Statistical analysis was performed using a Student’s t-test. *, P-value<0.05; **, P-value<0.01.