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. 2017 Nov 8;163(11):1680–1691. doi: 10.1099/mic.0.000558

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Fig. 2. — ClaR regulates A. tumefaciens biofilm formation. (a) A. tumefaciens quantitative biofilm formation on PVC coverslips after 48 h static growth at 28 °C. Adherent biomass quantified by staining with crystal violet (CV). CV absorbance quantified by absorbance at 600 nm (A₆₀₀). In parallel, the OD₆₀₀ of planktonic culture was determined. CV absorbance was normalized to culture growth by calculating the A₆₀₀/O_D600 ratio. Values are the result of two independent biological replicates consisting of three technical replicates each. Error bars: ±1 sd. (b) 48 h PVC coverslip biofilms quantified as described in Fig. 2(a). claR Annot.: 750 bp claR annotated in NCBI database. claR Ext.: annotated claR plus additional 84 bp added to 5′ end of gene. Dash represents strain without plasmid introduced. Values are the results of two independent biological replicates consisting of three technical replicates each. Error bars: ±1 sd.