Figure 3.

V gene diversity in the combinatorial TRG/TRD repertoire of different TCRγδ+ T-cell subsets from young and elderly individuals. V-gene usage per subset visualized in bar graphs indicating a diverse repertoire in naive and effector subsets of both young and elderly, with skewing toward Vγ2 and Vγ9 in memory populations (A). Naive populations showed diversity in Vδ usage with Vδ1 dominance in young and elderly. Young individuals showed relatively higher Vδ2 usage in especially the memory and effector subsets, whereas elderly individuals showed clear Vδ1 dominance in all subsets, except for effector memory cells (B). Median V-gene frequencies of productive sequences with SD bars were indicated in bar graphs for young (N = 11) and elderly (N = 12) individuals. Color legends are indicated in the figure. Statistical significance was tested using the Mann–Whitney U-test. Level of significance is indicated in the plots: *p < 0.05; **p < 0.01; ***p < 0.0001.