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. 2018 Mar 6;9:287. doi: 10.3389/fpls.2018.00287

Table 4.

Changes in TFAs content (mg g-1 DW), FA composition (% mol), and unsaturation (DBI) of coffee beans from C. arabica cv. Icatu collected at 25°C, and within the ranges of 30–35 and 36–40°C (diurnal temperatures).

Diurnal temperature at bean harvest
Parameter [CO2] (μL L-1) 25°C 30–35°C 36–40°C
TFA 380 58.6 ± 0.1 aA 58.2 ± 2.0 aA 53.7 ± 1.9 aA
(mg g-1 DW) 700 56.4 ± 2.5 aA 53.0 ± 1.5 aA 56.2 ± 1.8 aA
C16:0 380 35.2 ± 0.1 aA 35.3 ± 1.8 aA 32.6 ± 1.3 aB
(% mol) 700 35.8 ± 0.1 aA 34.8 ± 1.1 aA 36.3 ± 0.7 aA
C18:0 380 6.90 ± 0.30 bA 7.00 ± 0.40 bA 8.30 ± 0.30 aA
(% mol) 700 7.50 ± 0.40 aA 7.70 ± 0.20 aA 8.00 ± 0.20 aA
C18:1 380 9.80 ± 0.20 aA 9.50 ± 1.10 aA 10.40 ± 0.40 aA
(% mol) 700 9.80 ± 0.50 aA 9.40 ± 0.40 aA 8.90 ± 0.50 aA
C18:2 380 43.4 ± 0.1 aA 43.4 ± 0.2 aA 42.4 ± 0.7 aA
(% mol) 700 41.5 ± 0.4 aA 41.3 ± 1.1 aB 41.3 ± 0.8 aA
C18:3 380 1.00 ± 0.10 aA 0.80 ± 0.20 aA 1.10 ± 0.10 aA
(% mol) 700 1.00 ± 0.00 aA 1.10 ± 0.10 aA 1.30 ± 0.00 aA
C20:0 380 2.40 ± 0.10 aA 2.40 ± 0.00 aB 3.00 ± 0.20 aA
(% mol) 700 2.50 ± 0.10 bA 3.40 ± 0.20 aA 2.60 ± 0.30 bA
C20:1 380 0.310 ± 0.010 bB 0.550 ± 0.110 aB 0.550 ± 0.070 aA
(% mol) 700 0.570 ± 0.030 abA 0.770 ± 0.090 aA 0.460 ± 0.060 bA
C22:0 380 0.680 ± 0.100 aA 0.660 ± 0.220 aA 1.060 ± 0.030 aA
(% mol) 700 0.690 ± 0.020 aA 1.020 ± 0.070 aA 0.740 ± 0.120 aA
C24:0 380 0.210 ± 0.020 abA 0.130 ± 0.060 bA 0.270 ± 0.010 aA
(% mol) 700 0.190 ± 0.020 aA 0.230 ± 0.020 aA 0.180 ± 0.030 aA
DBI 380 2.20 ± 0.00 aA 2.20 ± 0.10 aA 2.20 ± 0.10 aA
700 2.10 ± 0.00 aA 2.00 ± 0.10 aA 2.00 ± 0.00 aA

For each parameter, the mean values ± SE (n = 4, performing two replicates of four plants) followed by different statistical indexes express significant differences between temperatures for the same CO2 treatment (a, b), or between CO2 treatments within each temperature (A, B). Although a few mean differences were observed, the two-way ANOVA only depicted significant changes [CO2] for C20:1. DBI = [%monoenes + 2 × %dienes + 3 × %trienes9/%saturated FAs] (Mazliak, 1983). Traces of C14:0, C15:0, and C16:1 (<0.1% mol) were also found.