Fig. 2.

Ability of various MAT/metK genes to complement the AdoMet dependence of ∆metK E. coli. (a) Growth curves in LB broth. The nine strains are all in the same background (E. coli MOB1490), and all with a pUC57-derived plasmid carrying the sequence shown in Fig. 1(b) and a gene for each of the nine AdoMet synthetases shown in Fig. 1(a). (b) Growth curves as in (a), but in MOPS minimal glucose medium. (c) Growth curves as in (b), but showing only the EcoMetK strain, where the overnight culture was inoculated with a colony from an LB agar plate (‘stock’) or from a minimal glucose plate (‘plate’).