Fig. 5.

Effect of iron limitation and haemin on growth of the S. aureus RN6390 and essC mutant strains. (a) S. aureus RN6390 and the essC deletion strain were grown with shaking in either TSB medium or iron-depleted TSB medium (prepared using Chelex-100 to remove all divalent cations followed by re-introduction of Zn²⁺, Mn²⁺, Mg²⁺ and Ca²⁺ as described in the Methods) that had been supplemented with the indicated iron source. FAC, ferric ammonium citrate; hhg, human haemoglobin. (b) Growth of S. aureus RN6390 and the essC deletion strain in TSB or TSB supplemented with 1 or 10 µM haemin, as indicated. Growth was monitored over 18 h in 96-well plates (200 µl volume). Error bars are ±sd, n=3.