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. 2017 Nov 22;163(12):1851–1863. doi: 10.1099/mic.0.000580

Fig. 1.

Fig. 1.

EsaB is not a transcriptional regulator. (a) The ess locus in S. aureus RN6390. Genes encoding essential secretion components are shaded in grey, secreted proteins in blue and a cytoplasmic antitoxin in yellow. The regions analysed by RT-PCR are indicated. (b) Predicted subcellular locations of Ess-encoded components. cw–cell wall, cm–cytoplasmic membrane. (c) RT-PCR analysis of esxA (region 1) and esxC/B (region 2) from the RN6390 and isogenic esaB and esxA mutant strains. Shading is as for Fig. 1(a) with essential secretion components in grey, secreted proteins in blue and a cytoplasmic antitoxin in yellow. Equivalent amounts of mRNA from each strain were used to generate cDNA. RT: reverse transcriptase. (d) Total mRNA counts of ess genes from RNA-Seq analysis of RN6390 and the esaB mutant strain. RPKM – reads of transcript per kilobase per million of mapped reads.