Table 2. Genes differentially regulated (>log 2 fold) in the RN6390 esaB deletion mutant, sorted by ascending fold change (FC).
Genes highlighted in grey are also differentially regulated in the essC deletion strain. The column on the right shows the fold change (FC) of the same gene in the essC dataset where ns indicates no statistically significant change in expression level relative to the same gene in the wild-type dataset.
| Locus ID | Gene name | FC in esaB mutant | Proposed function | FC in essC mutant |
|---|---|---|---|---|
| Downregulated genes | ||||
| SAOUHSC_00986 | sspC | −23.7 | Cysteine protease | ns |
| SAOUHSC_00988 | sspA | −22.3 | Glutamyl endopeptidase | ns |
| SAOUHSC_00987 | sspB | −20.8 | Cysteine protease | ns |
| SAOUHSC_01573 | – | −19.0 | Unknown, hypothetical protein | ns |
| SAOUHSC_01941 | splB | −18.8 | Serine protease SplB | −4.3 |
| SAOUHSC_02971 | aur | −17.1 | Zinc metalloproteinase aureolysin | ns |
| SAOUHSC_01942 | splA | −16.4 | Highly specific serine protease specific to S. aureus | −5.4 |
| SAOUHSC_02680 | narH | −15.7 | Nitrate reductase subunit beta | ns |
| SAOUHSC_01944 | – | −14.3 | Unknown, hypothetical protein | −4.5 |
| SAOUHSC_02681 | narG | −14.3 | Nitrate reductase subunit alpha | ns |
| SAOUHSC_01121 | hla | −13.5 | α-hemolysin | −4.1 |
| SAOUHSC_02241 | lukF | −13.0 | Unknown, hypothetical protein | −3.3 |
| SAOUHSC_02163 | hlb | −12.3 | β-hemolysin | ns |
| SAOUHSC_01938 | splD | −12.2 | Serine protease SplD | −4.3 |
| SAOUHSC_02679 | narJ | −12.2 | Nitrate reductase subunit delta | ns |
| SAOUHSC_02671 | narK | −11.6 | Putative nitrate transporter | ns |
| SAOUHSC_02455 | lacA | −11.0 | Galactose-6-phosphate isomerase subunit LacA | nd |
| SAOUHSC_01530 | – | −10.9 | Hypothetical phage protein | ns |
| SAOUHSC_01542 | – | −10.9 | Unknown, SNF2 family protein | ns |
| SAOUHSC_01535 | – | −10.9 | Phage capsid protein | ns |
| SAOUHSC_02240 | hlb | −10.5 | Truncated β-hemolysin | ns |
| SAOUHSC_02243 | lukG | −10.4 | Leukocidin like toxin | −4.5 |
| SAOUHSC_02685 | nirR | −10.3 | Unknown, hypothetical protein | ns |
| SAOUHSC_01939 | splC | −10.3 | Serine protease SplC | −3.2 |
| SAOUHSC_01937 | – | −10.3 | Unknown, hypothetical protein | −2.8 |
| SAOUHSC_02970 | argR | −8.8 | Arginine repressor family protein | ns |
| SAOUHSC_00113 | adhE | −8.6 | Bifunctional acetaldehyde-CoA/alcohol dehydrogenase | ns |
| SAOUHSC_00051 | plc | −8.1 | 1-phosphatidylinositol phosphodiesterase | −2.5 |
| SAOUHSC_00898 | argH | −6.7 | Argininosuccinate lyase | ns |
| SAOUHSC_02684 | nasD | −6.6 | Assimilatory nitrite reductase [NAD(P)H] large subunit | ns |
| SAOUHSC_02709 | hlgC | −6.5 | γ-hemolysin component C precursor | −1.8 |
| SAOUHSC_02682 | nasF | −6.4 | Uroporphyrin-III C-methyltransferase | ns |
| SAOUHSC_02462 | – | −6.4 | Unknown, hypothetical protein | ns |
| SAOUHSC_00401 | – | −6.3 | Putative exported protein | −1.6 |
| SAOUHSC_01950 | epiD | −6.3 | Flavoprotein | ns |
| SAOUHSC_01936 | splE | −6.3 | Serine protease SplE | −3.3 |
| SAOUHSC_02454 | lacB | −6.3 | Galactose-6-phosphate isomerase subunit LacB | −3.4 |
| SAOUHSC_00899 | argG | −6.2 | Argininosuccinate synthase | ns |
| SAOUHSC_02108 | ftn | −6.1 | Ferritin | ns |
| SAOUHSC_00368 | – | −6.1 | Unknown, hypothetical protein | ns |
| SAOUHSC_00411 | – | −5.9 | Unknown, hypothetical protein | −2.2 |
| SAOUHSC_01951 | epiC | −5.8 | Epidermin biosynthesis protein EpiC | ns |
| SAOUHSC_02683 | nasE | −5.6 | Assimilatory nitrite reductase [NAD(P)H] small subunit | ns |
| SAOUHSC_01935 | splF | −5.3 | Serine protease SplF | −2.7 |
| SAOUHSC_02452 | lacD | −5.2 | Tagatose 1,6-diphosphate aldolase | −2.6 |
| SAOUHSC_01953 | epiA | −5.2 | Gallidermin superfamily EpiA protein | ns |
| SAOUHSC_02941 | nrdG | −4.9 | Anaerobic ribonucleotide reductase activating protein | ns |
| SAOUHSC_00717 | saeP | −4.7 | Putative lipoprotein | −1.4 |
| SAOUHSC_01990 | glnQ | −4.6 | Glutamine transport ATP-binding protein | ns |
| SAOUHSC_02557 | yut | −4.5 | Putative urea transporter | ns |
| SAOUHSC_01949 | epiP | −4.4 | Intracellular serine protease | ns |
| SAOUHSC_00120 | capG | −4.4 | UDP-N-acetylglucosamine 2-epimerase | ns |
| SAOUHSC_01952 | bsaB | −4.4 | Lantibiotic epidermin biosynthesis protein EpiB | ns |
| SAOUHSC_03015 | hisZ | −4.4 | ATP phosphoribosyltransferase regulatory subunit | ns |
| SAOUHSC_00119 | capF | −4.4 | Capsular polysaccharide biosynthesis protein CapF | ns |
| SAOUHSC_02463 | hysA | −4.3 | Hyaluronate lyase | ns |
| SAOUHSC_02453 | lacC | −4.1 | Tagatose-6-phosphate kinase | −2.2 |
| SAOUHSC_00608 | adh1 | −4.1 | Alcohol dehydrogenase | ns |
| Upregulated genes | ||||
| SAOUHSC_02767 | opp-1A | 4.0 | Peptide ABC transporter substrate-binding protein | 2.6 |
| SAOUHSC_02655 | – | 4.2 | Unknown, hypothetical protein | 6.3 |
| SAOUHSC_01292 | – | 4.4 | Putative DNA-binding protein | ns |
| SAOUHSC_00130 | isdI | 4.4 | Heme-degrading monooxygenase IsdI | 5.7 |
| SAOUHSC_00176 | – | 4.5 | Extracellular solute-binding protein | ns |
| SAOUHSC_02435 | sfaA | 4.5 | Putative transporter | 6.7 |
| SAOUHSC_02799 | sarT | 4.6 | Staphylococcal accessory regulator | ns |
| SAOUHSC_02432 | – | 4.8 | Unknown, hypothetical protein | 6.2 |
| SAOUHSC_02245 | – | 4.9 | Unknown, hypothetical protein | 6.5 |
| SAOUHSC_00652 | fhuA | 5.1 | Ferrichrome ABC transporter ATP-binding protein FhuA | 7.0 |
| SAOUHSC_00071 | sirC | 5.3 | Involved in staphyloferrin B transport into the cytoplasm | 4.6 |
| SAOUHSC_00131 | – | 5.3 | Putative membrane protein | 6.1 |
| SAOUHSC_02821 | – | 5.8 | Putative membrane protein | ns |
| SAOUHSC_02719 | – | 6.2 | ABC transporter ATP-binding protein | 5.5 |
| SAOUHSC_01920 | – | 6.3 | Putative lipoprotein | ns |
| SAOUHSC_02428 | htsB | 6.3 | Heme transport system permease HtsB | 5.4 |
| SAOUHSC_00974 | – | 6.4 | Unknown, hypothetical protein | ns |
| SAOUHSC_01081 | isdA | 6.5 | Iron-regulated heme-iron binding protein | 5.4 |
| SAOUHSC_00072 | sirB | 6.5 | Involved in staphyloferrin B transport into the cytoplasm | 7.4 |
| SAOUHSC_02554 | fhuD2 | 6.5 | Ferric hydroxamate receptor 1 FhuD2 | 6.8 |
| SAOUHSC_01090 | – | 6.7 | Unknown, hypothetical protein | 3.9 |
| SAOUHSC_00973 | – | 7.9 | Unknown, hypothetical protein | ns |
| SAOUHSC_01086 | isdF | 8.5 | ABC permease IsdF | 6.1 |
| SAOUHSC_01085 | isdE | 8.6 | Heme-receptor lipoprotein IsdE | 5.6 |
| SAOUHSC_01089 | isdG | 8.7 | Heme-degrading monooxygenase IsdG | 4.7 |
| SAOUHSC_01087 | – | 8.9 | Iron compound ABC transporter permease | 6.3 |
| SAOUHSC_01082 | isdC | 8.9 | Heme transporter IsdC | 5.5 |
| SAOUHSC_00748 | sstC | 9.6 | Ferrichrome ABC transporter ATP-binding protein SstC | 9.1 |
| SAOUHSC_00545 | sdrD | 10.0 | Fibrinogen-binding protein SdrD | ns |
| SAOUHSC_02246 | fhuD1 | 10.0 | Iron compound ABC transporter FhuD1 | 8.0 |
| SAOUHSC_00972 | – | 10.1 | Unknown, hypothetical protein | ns |
| SAOUHSC_01088 | srtB | 10.2 | Sortase StrB | 6.2 |
| SAOUHSC_00747 | sstB | 10.4 | Ferrichrome ABC transporter permease SstB | 9.0 |
| SAOUHSC_00070 | sarH1 | 11.2 | Unknown, hypothetical regulatory-like protein | ns |
| SAOUHSC_02430 | htsA | 11.2 | Heme transport system lipoprotein HtsA | 10.5 |
| SAOUHSC_00746 | sstA | 11.9 | Ferrichrome ABC transporter permease SstA | 10.9 |
| SAOUHSC_01084 | isdD | 13.3 | ATP-hydrolysing and heme-binding protein IsdD | 6.2 |
| SAOUHSC_00074 | sirA | 13.6 | Receptor component of staphyloferrin B | 16.3 |
| SAOUHSC_01514 | – | 15.6 | Unknown, hypothetical protein | ns |
| SAOUHSC_02232 | – | 16.7 | Hypothetical phage protein | ns |
| SAOUHSC_02084 | – | 17.7 | Phage repressor protein | ns |
| SAOUHSC_02218 | – | 25.9 | Unknown, hypothetical protein | ns |
| SAOUHSC_00069 | spa | 51.5 | Protein A | ns |