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. 2018 Mar 6;9:194. doi: 10.3389/fphar.2018.00194

FIGURE 1.

FIGURE 1

Chemotherapeutics promoted SIRT6 expression in HCC cells. The mRNA (A) and protein (B) levels of SIRT1 to SIRT7 were examined in Huh-7 cells treated with chemotherapeutic agents (doxorubicin, cisplatin, and sorafenib) for 48 h by using qPCR and Western blotting analysis. β-Actin was used as an internal control for qPCR. GAPDH protein expression was used as a loading control for Western blotting analysis. ∗P < 0.05 vs. control. (C) The mRNA and protein expression levels of SIRT6 were examined by using qPCR and Western blotting analysis. Huh-7 and SK-Hep-1 cells were exposed to the doxorubicin (1 μg/mL), cisplatin (2 μg/mL), and sorafenib (4 μg/mL) for 48 h, respectively. The mRNA expression level of β-actin was used as an internal control for qPCR analysis and GAPDH protein expression was used as an internal control for Western blotting analysis. ∗P < 0.05 vs. control. (D) The protein expression level of SIRT6 was tested in Huh-7 and SK-Hep-1 cells treated with various concentrations of the three chemotherapeutics for 48 h by using Western blotting analysis.