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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2018 Feb 2;117:99–109. doi: 10.1016/j.freeradbiomed.2018.01.023

Figure 3. Overexpression of ECSOD and knockdown of xCT suppresses the invasive ability of aggressive PC3.

Figure 3

(A) Western blots display comparatively lower ECSOD (~31 kDa) and greater xCT (~55 kDa) expressions in PrEC, PC3, and WPMY1 cell lines. (B). Western blots indicate an increase of ECSOD expression in PC3 and WPMY1 cells after treatment with the DNA methylation inhibitor, 5-Aza-dC, for 96 h. (C) Western blot shows an up-regulation of Nrf2 and xCT expression in PC3 and WPMY1 cells relative to the PrEC cells. (D) Cell invasive ability of PC3 after treatment with SOD and the xCT inhibitor (SASP) for 24 h. (E) Western blot indicates an overexpression of ECSOD in the media and in cell lysates with AdhSOD3 (300 MOI) treatment and a down-regulation of xCT protein by siRNA (siRNA xCT). (F) Cell invasive ability of PC3 cells after overexpression of ECSOD in the media and cells, and with concurrent inhibition of xCT expression. AdEmpty; adenovirus empty vector. 300 MOI was used for AdEmpty and AdhSOD3. *p-value ≤ 0.05 compared with the control.